The France international said in the week that he did not speak with Djukic regularly and that their relationship was "not good".
He also suggested that there were no players within the Valencia dressing room willing to stand up to the Serbian boss.
However, following what the club describe as "disrespectful public statements", Rami has been withdrawn from the squad ahead of their clash at the Estadio Nuevo Los Carmenes on Wednesday.
A statement on the club's official website read: "Valencia CF manager Miroslav Djukic, with the full backing of the club, has made the decision to remove Adil Rami from the squad for the game against Granada.
"Disrespectful public statements by the player towards the club and his team-mates, made without the permission or knowledge of Valencia CF, are the reason for Rami's removal.
"Valencia CF and the values of the club come before personal interests and any other matter."
Chelsea loanee Oriol Romeu will take Rami's place in the squad for the match, which sees 11th face 12th.
Rami was sent off after 10 minutes in Valencia's recent UEFA Europa League defeat to Swansea City, before they bounced back with a 3-1 win over Sevilla on Sunday.
However, Djukic's side have lost three of their last five La Liga matches.
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